Work Rambles, no formatting

Today has not gone the way I had planned. I woke up with a splitting headache and it felt like I was moving though a dense fog. I still got up for work and made the plans for the day in my head. I was going to spin down the cultures I had shaking from overnight and I was going to purify protein from those expressed cells. After that I was going to do a liposome experiment with the newly purified protein. There was only one problem, a problem I only realized after I had gotten to work. I have to transfer the protein to a different buffer. This process takes an hour or two to complete, so I wasn't going to be able to get to the liposome experiments like I wanted to. I pretty much head desked as soon as I got to my desk. So I spun down those cultures, put them in the freezer, froze a few other things and I left. Maybe I could sleep off some of this crude. I did sleep when I got home, slept for 4 hours. I didn't wake up until my stomach told me too. My head still isn't clear, and I would really like to go back to bed, but I have a meeting with the boss. I don't have much to say, so I probably could have cancelled it, but I want some feed back from him on something. I have hit a few blocks and I don't know which things I should be focusing on. I need to get DNA from over 1100 genes to ship to an institution in New York. I have very few plates of DNA already that I could just sent what we have, but then we have no stock, or I can make fresh stocks and have the proper amount of DNA sent to them. Either way, I don't have much in the lines of the DNA that I need to send. I had such a hard time getting the DNA from the 96-well minipreps, and most of the DNA that we did have, it has already been shipped to someone else or was split up among people. The boss also never got back to me on a plasmid I found that was for an idea of what he was wanting to do. I don't like buying things without his okay. It may be something that I need to get over because he does tend to be "yes, we want to do it this way, buy it." more often than saying no. But he does have a tendency to change his mind after something was ordered. So, I just want the okay first. I want to tell him how I plan on setting up the liposome experiments. Trying only one protein at a time with different conditions rather than trying multiples with only one condition. The way I am doing it now it makes it kind of difficult to conclude anything. I have to go back to old data sets and be like, well, it kind of looks like there could be a change, but this experiment was done a few weeks ago and some random condition could have been different. So, no I will do all the conditions at the same time. One with have no added substrate and be my control and the other there will be different substrates he wants to try. I have two of the substrates made up already, the third I think I know what he wants to use, but I don't know the best concentration that he might want to use to see if it helps or hurts the binding of the proteins. I really just want to make sure that I am doing what he wants. I lost my first job in less than 8 months, and not for not trying. I just don't want to be the person who is expendable and can be cut from the grant funding. I know I am blessed having this job, I don't want to let him down, but somedays it feels like I am doing to much and stress gets to me. I try my hardest to get things done, but it just doesn't always happen. Hopefully, tomorrow I can get some stuff set up and have things ready to go for first thing Monday morning to rocket off with the experiments I already have planned and then some. Though, I have a feeling that most of it will just be the single liposome experiment and making a lot of DNA. *head desk* And I haven't even gotten to touch my short fanfic today. Bah