What's wrong with me today?

[sammyphoenix]

Seriously, I am so tired of failing at the work that I do. If it isn't one thing it is another. I have been working for several week to get my lipid blots working again. I have tried new proteins, new lipids, new buffers, new paper, and nothing seems to give me the results that I had the previous month with the same proteins. Grrr, it is making me mad! It makes me feel like a big screw up at work and I have no idea how to fix it. I'm hoping the ones I am setting up today will work. I have fresh proteins and am trying the other end of the binding paper as my boss suggested to see if the paper just isn't binding to the lipids. If this doesn't work tomorrow I may scream. I guess a good thing is that I have a meeting set up tomorrow afternoon to meet with my boss so we can go over more troubleshooting methods. I just want my stuff to work.

I also feel terrible because I had to do an extra purification step with one of my proteins. The blots with this protein always has a lot of background binding that I have not been able to get rid of. Boss suggested that the next time I do a purification that I do a second purification on the first one. I did that today. The first purification looked okay. I did not set it up in the factions like I usually do, but the liquid did have a tent of green so I am fairly sure that I had protein there. I ran it through a second time and collected factions. Nothing. My factions were crystal clear. >.< Where did my protein go!

Why can't any of my work just work!